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Enhancement of protease production from Rhizomucor miehei by mutagenesis with ethyl methanesulfonate, ultraviolet, and microwaves- a preliminary study
Title Enhancement of protease production from Rhizomucor miehei by mutagenesis with ethyl methanesulfonate, ultraviolet, and microwaves- a preliminary study
Authors Houthail Alahmad Aljammas, Sabah Yazji, Abdulhakim Azizieh
Source title Bioresource Technology Reports
ISSN 2589-014X
Q Q1
Link

https://www.sciencedirect.com/science/article/abs/pii/S2589014X22003449
Abstract Rhizomucor miehei protease has been emerging as a potent substitute for the more expensive calf rennet. Enzyme biosynthesis could be improved by using random mutagenesis techniques. In this study, the fungal spore suspension was treated with different concentrations of ethyl methanesulfonate (EMS) (20–80 μg/mL) for different incubation periods (10–60 min). UV-mutagenesis was carried out at different distances (5–25 cm) from the exposure source (253 nm) for different exposure periods (5–25 s). Microwaves (MW) irradiation was done at 2450 MHz for different exposure periods (90–210 s). The best results, in terms of milk-clotting activity (MCA) and milk-clotting activity to proteolytic activity ratio (MCA/PA), were obtained with the protease secreted by the MW-induced mutant (150 s), followed by UV (10 cm*25 s), and then EMS (35 μg/mL*20 min) induced mutants. MW150 mutant presented 3.68 and 2.18 folds improvement in MCA and MCA/PA ratio. Only MW-induced mutant was stable after 8 generations


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