Cutaneous leishmania is a common medical problem in Syria, which has become yet more widespread in most of the Syrian governorates after the current war. An effective vaccine is needed to prevent a large scale spread of leishmaniasis in the country. In this regard, protein vaccination has shown promising prospects of creating this much -needed vaccine. Thiol Specific Antioxident (TSA) plays a fundamental role in the life of parasites. Its gene provides protective properties in the form of DNA vaccines against infections by many species of parasites and bacteria. So far, the presence of this gene in the genome of the Syrian strains of L. tropica has not been proven. This study aimed to investigate the presence of the gene in the Syrian strain of Leishmania tropica promastigotes, clone and compare with the TSA sequence in other Leishmaniaspp. We isolated genomic DNA from these parasites, designed primers for amplification of the gene encoding this protein, and standardized the PCR conditions .