Aim: Iron overload can have detrimental health effects. Angiotensin II is involved in body iron storage. Therefore, the current study aimed to investigate angiotensinconverting enzyme inhibitor "lisinopril" efficacy in reducing body iron content under iron overload conditions, using an experimental model induced by ferrous sulfate in rats. Materials and methods: 23 male Albino Wistar rats, divided into three groups; control: distilled water 1ml/day, Iron overload: ferrous sulfate 30 mg/kg/day, treatment with Lisinopril: ferrous sulfate + Lisinopril 1 mg/kg/day intraperitoneal injections daily for 14 days. Body iron stores were evaluated by hepatic and serum iron assay. Results: Ferrous sulfate increased body iron storage as evidenced by elevated serum and hepatic iron levels. Lisinopril did not cause a significant reduction in iron parameters when injected daily for 14 days (P > 0.05). Conclusions: Angiotensin inhibition may not be beneficial in reducing body iron stores under iron overload conditions