The Curie point depth (CPD) has been established to enhance the determination of magnetic sources average bottom depth. In this study, the CPD and heat flow regime of the Eratosthenes Seamount in the Eastern Mediterranean were investigated in order to understand the relationship between them. When CPD and heat flow values are determined together, we can understand the types and processes of geothermal resources formation. CPD and heat flow were obtained by applying the spectral analysis method to magnetic data, which was obtained from the Earth Magnetic Anomaly Grid (EMAG2). The result shows that CPD is approximately 22 km across the Seamount and is approximately 9 km in the northern part of the Eratosthenes Seamount near Cyprus. The heat flow regime manifested from CPD is about 95 mW/m2 in the investigated region, where the average thermal conductivity value (k) is considered to be 2.5 W/m ◦C. The heat flow regime is characterized by an increase in the northward part of the Eratosthenes Seamount and decreases towards the south.