Fusarium is a worldwide distributed fungal genus and includes different phytopathogenic species which are able to infect cereals among other crops. This study was carried out to identify and characterize the isolates of Fusarium spp. in the Gibberella fujikuroi species complex (Liseola section) through morphological and molecular approaches. A total of 105 isolates of Fusarium spp. were recovered from crown and roots of wheat plants, showing typical symptoms, collected from four Syrian provinces during 2017-2018. Seventeen isolates in the G. fujikuroi species complex were identified as F. proliferatum, F. verticillioides and F. andiyazi based on morphological characteristics, and sequencing a partial translation elongation factor 1-alpha (TEF1-α) gene. The pathogenicity test showed that all recovered isolates of these three species were pathogenic, with low to moderate severity, on durum wheat seedlings under laboratory conditions. To our knowledge, this is the first report of F. andiyazi causing crown and root rot on wheat